![]() Emission from RLuc and citrine was measured simultaneously at 465 and 535 nm in a DTX-800 plate reader (Beckman Coulter). Coelenterazine was added to a final concentration of 2 μ m immediately before measuring BRET. Inhibitors were added 5 min before the indicated treatments. ![]() 100 μl was added to 96-well white plates and treated for 5 min at room temperature with the indicated treatments. After centrifugation at 20,000 × g for 10 min, the supernatant was removed and diluted to the desired volume.
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